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Antibacterial Activity of Extracts from Dried and Fresh Herbal Plant (Phyllanthus amarus) Against Pathogens Causing Acute Hepatopancreatic Necrosis Disease (Ahpnd) in White Leg Shrimp (Litopenaeus vannamei) at Thua Thien Hue Province, Vietnam | Abstract

➣ Original Article

➣ Asp Biomed Clin Case Rep. 2019 Nov 22;2(3):120-128

Phuong TV1, Hai Yen PT2, Linh NQ1,3*

1Institute of Biotechnology, Hue University, Road No.10, Phu Vang, Thua Thien Hue, 530000, Vietnam

2Faculty of Fisheries, University of Agriculture and Forestry, Hue University, 102 Phung Hung St., Hue, 530000, Vietnam

3Hue University, 3 Le Loi St., Hue, 530000, Vietnam

Corresponding Author: Nguyen Quang Linh

Address: Institute of Biotechnology, Hue University, Road No.10, Phu Vang, Thua Thien Hue, 530000, Vietnam; E-mail: nguyenquanglinh@hueuni.edu.vn; phamthihaiyen@hueuni.edu.vn

Received date: 24 October 2019; Accepted date: 16 November 2019; Published date: 22 November 2019

Abstract

The study aimed to determine extract yield (%), antibacterial activity, and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of plant extracts from dried and fresh herbal plants (Phyllanthus amarus) against Vibrio parahaemolyticus strain causing acute hepatopancreatic necrosis disease (AHPND) in white leg shrimp (L. vannamei). The result showed that the extract yields of dry and fresh herbs reached 11.50% and 2.75%, respectively and the antibacterial activity of the two extracts both are good at concentrations from 250 to 1,000 mg/mL at the same bacterial density of 106 CFU/mL. Specifically, the diameter of the inhibition zone at 250; 500; 750 and 1,000 mg/mL concentration of dried herbal extracts reached 16.75±0.96; 18.50±1.29; 20.75±0.96 and 21.25±0.50 mm, while that of fresh herbal extracts reached 14.50±1.29; 16.25±0.50; 16.75±0.50 and 17.00±0.00 mm, respectively, with a statistically significant difference p<0.05. The result also showed that MIC values of dried and fresh extracts were defined at 125 mg/mL and 250 mg/mL, respectively and that MBC values of the extracts were 500 and 1,000 mg/mL respectively. The GC-MS analysis revealed that there were 19 natural compounds in the dried extract, in which Ethyl Linoleolate (C20H36O2) compound occupied the highest ratio (22.43 %), while 2.3-Dihydro-3.5-dihydroxy-6-methy-4H-pyran-4-one (C6H8O4) was the lowest (0.24 %).

Citation: Phuong TV, Hai Yen PT, Linh NQ. Antibacterial Activity of Extracts from Dried and Fresh Herbal Plant (Phyllanthus amarus) Against Pathogens Causing Acute Hepatopancreatic Necrosis Disease (Ahpnd) in White Leg Shrimp (Litopenaeus vannamei) at Thua Thien Hue Province, Vietnam. Asp Biomed Clin Case Rep. 2019 Nov 22;2(3):120-128.

Copyright © 2019 Phuong TV, Hai Yen PT, Linh NQ. This is an open-access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium provided the original work is properly cited.

Keywords: EMS; AHPND; Herbs; Extract; Phyllanthus amarus; MIC/MBC

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