ASPLORO OPEN ACCESS PUBLICATIONS
An international peer-reviewed open access journal presenting original research contributions and scientific advances in genetics, genomics and evolutionary biology including molecular biology and molecular evolution.
Background: Hodgkin Lymphoma (HL) represents a disease of successful outcome due to advances in modern medicine. A significant percentage of patients respond very well to treatment, achieving relapse free survival. However, despite appropriate treatments as many as 20% of these patients die of this disease. Risk stratification allows therapy to be selected based on specific prognostic indicators.
Procedure: A retrospective cohort study containing 25 pediatric classical HL cases were evaluated from the files of Miami Children’s Hospital Department of Pathology. The study aimed to analyze tumor-associated macrophages via CD68 immunohistochemistry in tissue obtained at the time of diagnosis. It studied the prognostic value of CD68+ histiocytes against a patient’s response to treatment and survival rates, as a possible correlation of this biomarker with outcomes.
Results: Higher levels of CD68+ macrophages was strongly correlated with a significant probability of relapsing from complete response (P=0.005), along with a greater likelihood of death from lymphoma (P=0.024). Furthermore, survival analysis demonstrated a decreased progression-free survival (P=0.001) and disease specific survival (P=0.023) when the microenvironment showed elevation of these macrophages.
Conclusions: The presence of an increased expression of CD68+ macrophages was found to be associated with a worse prognosis in a pediatric patient with HL. This study, establishes a new use for CD68, as a reliable immunohistochemical marker in pediatric patients with equivalent predictor outcomes as those reported in adult cases. This biomarker helps to identify those pediatric patients at higher risk of treatment failure, and thus provide the basis for individualized patient treatment.
Background: Neonatal cardiomyopathy is a rare disease that ranges from being asymptomatic to abruptly lethal and is not well characterized . We investigated the clinical features of five neonates with cardiomyopathy in our hospital to determine key clinical characteristics.
Methods: We retrospectively reviewed the records of five newborns who were diagnosed with cardiomyopathy between January 2000 and December 2018. The primary evaluation included reasons for diagnosis, underlying diseases, therapy, and turning point.
Results: Patients with hypertrophic cardiomyopathy (HCM) or left ventricular noncompaction (LVNC) were diagnosed on the basis of cardiac murmur, while the patient with dilated cardiomyopathy (DCM) was diagnosed on the basis of sucking failure. Underlying diseases included Noonan syndrome and LEOPARD syndrome. All patients had received β-blockers, and those with DCM and LVNC were also administered diuretics and angiotensin-converting enzyme inhibitors. The two patients with HCM underwent follow-up as out-patients. One patient with HCM died at 3 years old because of arrhythmia. The patient with DCM died due to heart failure 38 days after birth. The patient with LVNC exhibited severe heart failure after birth, requiring follow-up while considering heart transplantation.
Conclusions: Noonan syndrome and LEOPARD syndrome, which is RAS/MAPK-related diseases, should be considered in patients diagnosed HCM. Because heart failure progresses rapidly in patients with neonatal DCM and those with LVNC, planned therapy should include consideration of heart transplantation.
Background: Use of general anaesthesia in outpatient invasive procedures has increased, especially in the field of dermatology. Being uncooperative, children often require general anaesthesia. Especially since surgical skin operations are painful and lengthy, dictating the use of general anaesthesia.
Aim: The purpose of this study was to evaluate the safety, significant adverse events, and the complication rates related to general anaesthesia, when used among pediatric population underwent skin surgeries.
Methods: We conducted a retrospective cohort study in the form of randomly selected patient chart review, with a surgical code in the array of 8,539–16,782 for 2 years. We reviewed registers to document any unexpected admissions, adverse events or complications. Surgical outcomes and anaesthesia complications were reviewed by three anaesthesia consultants. We conducted Inter-rater reliability test analysis and per cent agreement to determine the level of agreement between raters.
Results: A total of 211 procedures were reported for 211 patients with 19 diagnoses. No adverse events related to anesthesia were recognized in any of those selected patients, apart from minor complications noticed in twelve patients (P value<0.03). Kappa value range between 0.78-1.00 (95% CI, 0.46809 to 1.00).
Conclusion: In case of proper deploying of staff experience, appropriateness of choice of surgical procedure, patient selection, and modern technology as non-dependent confounding variables. Pediatric-trained anesthesiologists can safely use general anaesthesia in dermatological invasive procedures without significant complications.
BioFAT is a bioproduct contained PUFAs, Se, Vitamin E and herb (Euphorbia thymifolia Burm (L.)). Data were conducted on experiments of added 2 and 4% of BioFAT into basic diets for post-weaning piglets for 3 weeks of feeding to improve growth performance and health status, 22.45% and 32.86% of daily gain which was a higher than controls. The study also showed that there is reduced feed consumption for kg of growth rate from 1.67 kg of control was down to 1.36; and 1.29 kg of feed consumption (FCR). Furthermore, BioFAT is also enhanced for piglets to resistance E.coli infection and rate of infections was lower in added 2 and 4% of BioFAT from 20% to 6.7 and 0% piglets diarrhea. Especially the practice has shown that diversity of Escherichia coli isolates were obtained from common host sources of fecal pollution and characterized by using repetitive extragenic palindromic (REP) PCR fingerprinting.
A 2-month-old had fever, decreasing urine output and progressing abdominal distension. His renal function showed mild deterioration from the normal limits and ultrasound abdomen showed right perinephric collection measuring 1.7x 0.8 cm (Panel A) with grade 1 hydronephrosis. Micturating cystourethrogram revealed extravasation of dye from right lower pelvicalyceal system and grade 5 vesicoureteral reflux (Panel B).